Differential expression between African-ancestry and White patients diagnosed with Triple-Negative Breast Cancer: EGFR, Myc, Bcl2 and β-Catenin as ancestry-associated markers

摘要: PurposeTriple-negative breast cancer (TNBC) has a higher incidence, younger age of onset, and more aggressive behavior in African-ancestry women. Biological disparities have been suggested as an important factor influencing the ancestry-associated TNBC discrepancy. In this study, we sought to identify differential gene protein expression between White patients, controlling for patients menopause status pathological staging at diagnosis. MethodsDifferential analyses (DGEA) were performed using RNA-sequencing data from The Cancer Genome Atlas (TCGA). Gene set enrichment analysis (GSEA) Ingenuity Pathway Analysis (IPA), with focus on network design, highlight candidate genes further validation through immunohistochemistry samples followed Portugal. ResultsWith 52 African-American 90 included, TCGAs corroborate that incidence (28.42% vs 11.89%, p<0.0001). Particularly, premenopausal stage II disease also significantly lower survival probability, comparing (log-rank p=0.019 0.0038, respectively). DGEA results suggest profile differences are associated than status. Hippo pathway cellular community sets downregulated, while is upregulated African-Americans, patients. Furthermore, MAPK when disease. Due their central role highly scored networks resulted IPAs EGFR, Myc Bcl2 selected immunohistochemistry. We included {beta}-Catenin study it consensually reported be required tumorigenesis. Although used experiments geographically culturally distinct, both groups mostly western-African ancestry and, interesting, matched. ConclusionsWe found patterns TNBCs, particularly or staging. Myc, {beta}-catenin matching distinct populations these markers being indicators TNBCs development.