Molecular characterization of begomoviruses and DNA satellites associated with okra leaf curl disease in Cameroon
作者： Walter N. Leke , Muhammad N. Sattar , Emilia B. Ngane , Jacob M. Ngeve , Anders Kvarnheden
DOI: 10.1016/J.VIRUSRES.2013.03.010
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摘要: Abstract Okra leaf curl disease (OLCD) is the most important viral of okra in West Africa. In this study, a complex begomoviruses and associated DNA satellites were identified symptomatic plants from southwestern Cameroon. Sequence analyses showed that two (Lik1 Njo5) infected with begomovirus being recombinant cotton Gezira virus (CLCuGeV) yellow crinkle (OYCrV). The genome shared highest nucleotide identity isolates CLCuGeV at 87.8% therefore considered to be member new species, Cameroon (OLCuCMV). One plant (Mue5) was by 95.8% identy CLCuGeV, while Lik1, Mue1 Njo5, showing 93.7% OYCrV. comparisons phylogenetic suggest these represent Cameroonian strains OYCrV (CLCuGeV-CM OYCrV-CM). Mixed infection OLCuCMV OYCrV-CM found plants. A betasatellite divergent alphasatellites also begomoviruses. as (CLCuGeB) 93.3% other African CLCuGeB. alphasatellites, herein named Alpha-1 Alpha-2, 97.3% 95.2% identity, respectively, alphasatellite (CLCuGeA) Burkina Faso (OLCuBFA). These collective results emphasize extent diversity among okra-infecting begomovirus–satellite complexes western
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sci-hub.se 参考文章(56)
Judith K. Brown, Phylogenetic Biology of the Bemisia tabaci Sibling Species Group Springer, Dordrecht. pp. 31- 67 ,(2009) , 10.1007/978-90-481-2460-2_2
L. Bigarré, M. Chazly, M. Salah, M. Ibrahim, M. Padidam, M. Nicole, M. Peterschmitt, C. Fauquet, J.C. Thouvenel, Characterization of a new begomovirus from Egypt infecting hollyhock (Althea rosea) European Journal of Plant Pathology. ,vol. 107, pp. 701- 711 ,(2001) , 10.1023/A:1011967232319
Ali M. Idris, Judith K. Brown, Molecular analysis of Cotton leaf curl virus-Sudan reveals an evolutionary history of recombination. Virus Genes. ,vol. 24, pp. 249- 256 ,(2002) , 10.1023/A:1015380600089
Judith K. Brown, The Molecular Epidemiology of Begomoviruses Food Products Press. pp. 279- 315 ,(2001) , 10.1201/9781482277890-13
Joseph Ndunguru, Nigel J Taylor, Jitender Yadav, Haytham Aly, James P Legg, Terry Aveling, Graham Thompson, Claude M Fauquet, Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues. Virology Journal. ,vol. 2, pp. 45- 45 ,(2005) , 10.1186/1743-422X-2-45
Roger P. Hellens, E. Anne Edwards, Nicola R. Leyland, Samantha Bean, Philip M. Mullineaux, pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Molecular Biology. ,vol. 42, pp. 819- 832 ,(2000) , 10.1023/A:1006496308160
J.K. Brown, Current status of Bemisia tabaci as a plant pest and virus vector in agro-ecosystems worldwide FAO plant Protection Bulletin. ,vol. 42, pp. 3- 32 ,(1994)
J. K. Brown, K. Z. Ahmed, D. K. Njualem, V. P. K. Titanji, J. N. E. Ramsell, W. N. Leke, A. Kvarnheden, J. P. Legg, V. N. Fondong, FTA technology facilitates detection and identification of begomoviruses from okra plants in Cameroon. 8th African Crop Science Society Conference, El-Minia, Egypt, 27-31 October 2007.. pp. 655- 660 ,(2007)
Muhammad Nouman Tahir, Imran Amin, Rob W. Briddon, Shahid Mansoor, The Merging of Two Dynasties—Identification of an African Cotton Leaf Curl Disease-Associated Begomovirus with Cotton in Pakistan PLoS ONE. ,vol. 6, pp. e20366- ,(2011) , 10.1371/JOURNAL.PONE.0020366
S. L. Shih, S. K. Green, W. S. Tsai, L. M. Lee, V. Levasseur, First report of a distinct begomovirus associated with okra yellow crinkle disease in Mali Plant Pathology. ,vol. 56, pp. 718- 718 ,(2007) , 10.1111/J.1365-3059.2007.01599.X