Measuring through the microscope: development and evolution of stereological methods.

摘要: SUMMARY Obtaining, by means of microscopy, meaningful measurements pertaining to spatial structures requires methods which allow three-dimensional quantitative information be derived from the reduced available on two-dimensional flat sections structure. The most powerful that effect are those stereology based mathematical principles. This paper reviews early invention these methods, sought solve practical problems, and their further evolution as more rigorous foundations were developed. It is demonstrated stereological essentially sampling newer trends provide new sound solutions old elusive such anisotropy or particle number size.