Automated Process of Q-PCR/Gene Expression

摘要: PCR-based fluorescent detection assays for the relative and quantitative measurement of gene expression, such as Taq Man™, LUX™, SYBR Green™, are currently in wide spread use due to their general applicability, low cost, reproducibility, accuracy, ease use. One current limitation PCR (Q-PCR) is lack a fully integrated high-throughput method genomic diagnostic applications. Here we report reliable system automated extraction RNA, first-strand cDNA synthesis, quality control measures, consecutive real-time amplification, primary data analysis. As described, this procedure utilizes commonly available reagents pre-packaged “kits” RNA extraction, first strand Q-PCR, liquid handling, capillary electrophoresis that generally applicable variety robotic platforms. (JALA 2004;9:128-34)