Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real-Time PCR by Use of the MagNA Pure LC and LightCycler Instruments

摘要: We report on the development of a fully automated real-time PCR assay for quantitative detection hepatitis B virus (HBV) DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument was used purification and preparation mixtures. Real-time performed LightCycler instrument. An internal amplification control devised as competitor introduced into at stage to permit monitoring sample adequacy. limit found be 200 HBV copies/ml, linear dynamic range 8 orders magnitude. When samples from European Union Quality Control Concerted Action Proficiency Panel 1999 were examined, results acceptable agreement concentrations panel members. In clinical laboratory evaluation 123 samples, significant correlation obtained by Roche Monitor test Cobas Amplicor analyzer within that system. conclusion, newly developed has markedly reduced hands-on time, permits adequacy, is suitable routine laboratory.