Combinatorial synthesis of MUC1 glycopeptides: polymer blotting facilitates chemical and enzymatic synthesis of highly complicated mucin glycopeptides.

摘要: The chemoselective polymer blotting method allows for rapid and efficient synthesis of glycopeptides based on a “catch release” strategy between solid-phase water-soluble supports. We have developed heterobifunctional linker sensitive to glutamic acid specific protease (BLase). general procedure consists five steps, namely (i) the glycopeptide containing BLase linker, (ii) subsequent deprotections release from resin, (iii) intermediates in presence polymers with oxylamino functional groups, (iv) sugar elongations using glycosyltransferases, (v) target platform by selective promoted hydrolysis. combined use chemical syntheses peptides enzymatic carbohydrates would greatly contribute production complicated glycopepti...