Novel synthesis of functional mucin glycopeptides containing both N- and O-glycans.

摘要: Progress of synthetic methods using ligation makes it possible to access larger and multifunctionalized biomolecules. Recently, sortase-mediated was used as a new complementary technique for construction peptide protein other methods. Staphylococcus aureus Sortase A (SrtA) is transpeptidase that recognizes C-terminal LPXTG motif proteins cleave between T G, subsequently transfers the acyl component nucleophile containing N-terminal oligo-glycines. Toward development multi- heteroglycosylated synthesis, we utilized SrtA-mediated preparation MUC1-related glycopeptide analogs having both N- O-glycans model compounds. To further improve this strategy, also demonstrated merits by means polymer-supported protocol. The present strategy will facilitate rapid large-scale synthesis multiply functionalized neoglycoprotein types convenient models investigation structure–function relationship.