Pure alkaline phosphatase, its preparation and use
作者： Keith May , Stephanie Jane Senior , Mohamed Mutwahar Gani
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摘要: The enzyme alkaline phosphatase having the following properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 (iii) activator: Mg ++ inhibitor: EDTA thermal stability: 45° C. or below (iv) optimum temperature: 35°-45° (v) pH 6-11 (vi) optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between 5.0 and 6.0. An antibody-enzyme conjugate process for purifying a reagent test kit using purified are also disclosed.
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