Long fragment de novo assembly using short reads

摘要: Techniques perform de novo assembly. The assembly can use labels that indicate origins of the nucleic acid molecules. For example, a representative set identified from initial reads overlap with seed be used. Mate pair information A sequence read aligns to an end contig lead using other mate pair, and used determine which branch extend, e.g., in external cloud or helper contig. kmer index include indicating origin each molecules kmer, memory addresses correspond index, position pairs includes kmer. Haploid seeds also determined polymorphic loci population.