Influence of Ionizing Radiation on Oxygen Profiles in Different Types of Multicellular Spheroids
作者： T. Nylén , H. Acker , B. Bölling , G. Holterman , J. Carlsson
DOI: 10.2307/3577709
关键词:
摘要: Human glioma (U-118 MG and U-138 MG), human colorectal adenocarcinoma (HT-29), thyroid carcinoma (HTh 7), hamster embryonic lung (V79-379A) spheroids were irradiated with either single doses of 16 or 40 Gy fractionated eight times 5 Gy. Oxygen profiles in the measured microelectrodes at different following irradiation, these then compared oxygen parallel cultured nonirradiated spheroids. No significant radiation-induced changes seen any within first few days after irradiation. The did not show increase tension even longer times; however, they growth inhibited, number S-phase cells was strongly suppressed. Increases occur HT-29 V79-379A but only appeared more than a week when degeneration had started. Histological decrease diameter that started to degenerate about Thus radiation therapeutic range not, for studied, produce rapid increases tension. When change occurred, it rather late probably consequence cell degeneration.
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sci-hub.se 参考文章(26)
J. Carlsson, J. M. Yuhas, Liquid-Overlay Culture of Cellular Spheroids Spheroids in Cancer Research. ,vol. 95, pp. 1- 23 ,(1984) , 10.1007/978-3-642-82340-4_1
R. E. Durand, R. M. Sutherland, Radiation Studies with Spheroids Spheroids in Cancer Research. ,vol. 95, pp. 103- 115 ,(1984) , 10.1007/978-3-642-82340-4_6
T. B. Constable, N. T. S. Evans, The Distribution of Oxygen Consumption Rates in Some Tissues before and after X-Irradiation Advances in Experimental Medicine and Biology. ,vol. 75, pp. 611- 617 ,(1976) , 10.1007/978-1-4684-3273-2_72
R. M. Sutherland, R. E. Durand, Growth and Cellular Characteristics of Multicell Spheroids Spheroids in Cancer Research. ,vol. 95, pp. 24- 49 ,(1984) , 10.1007/978-3-642-82340-4_2
H. Acker, G. Holtermann, J. Carlsson, T. Nederman, Methodological aspects of microelectrode measurements in cellular spheroids. Advances in Experimental Medicine and Biology. ,vol. 159, pp. 445- 462 ,(1983) , 10.1007/978-1-4684-7790-0_38
C. von Sonntag, The chemical basis of radiation biology ,(1987)
H. Acker, T. Nederman, T. Nylén, J. Carlsson, G. Holtermann, Influence of Glucose and Buffer Capacity in the Culture Medium on Growth and pH in Spheroids of Human Thyroid Carcinoma and Human Glioma Origin Cancer Research. ,vol. 47, pp. 3504- 3508 ,(1987)
R. M. Sutherland, W. Mueller-Klieser, B. Sordat, J. Bamat, H. Gabbert, B. Bourrat, Oxygenation and differentiation in multicellular spheroids of human colon carcinoma. Cancer Research. ,vol. 46, pp. 5320- 5329 ,(1986)
R.P. Hill, Sensitizers and radiation dose fractionation: results and interpretations. International Journal of Radiation Oncology Biology Physics. ,vol. 12, pp. 1049- 1054 ,(1986) , 10.1016/0360-3016(86)90223-3
J. Carlsson, K. Nilsson, B. Westermark, J. Pontén, C. Sundström, E. Larsson, J. Bergh, S. Påhlman, C. Busch, V. P. Collins, Formation and growth of multicellular spheroids of human origin. International Journal of Cancer. ,vol. 31, pp. 523- 533 ,(1983) , 10.1002/IJC.2910310502