Influence of Glucose and Buffer Capacity in the Culture Medium on Growth and pH in Spheroids of Human Thyroid Carcinoma and Human Glioma Origin

摘要: Abstract pH gradients were measured with microelectrodes in cellular spheroids of human glioma (U-118 MG) and thyroid carcinoma (HTh7) origin. decreased outside the then continuously when electrode was moved through spheroid towards center. The lowest values inside range 6.7–6.8 grown under standard conditions F10 medium. When medium stronger buffer capacity (Dulbecco9s minimum essential or Locke9s) used, both types less steep. Less steep also obtained concentration glucose lowered to 0.1 g/liter In case HTh7 low culture seemed toxic because growth rate increased cultured giving higher central (high concentration). No such growth-stimulating effects could be seen for U-118 MG spheroids. retarded they very high (10 g/liter). thickness viable cell layer g/liter. A decrease observed at a