Distribution and characterisation of CCK containing enteroendocrine cells of the mouse small and large intestine

摘要: There is general consensus that enteroendocrine cells, EEC, containing the enteric hormone cholecystokinin (CCK) are confined to small intestine and predominate in duodenum jejunum. Contrary this, EEC express gene for CCK have been isolated from large of mouse there evidence contain CCK-like immunoreactivity colon. However, human rat colons do not cells. In current study, we use immunohistochemistry investigate peptide presence endocrine PCR identify cck transcripts chromatography forms intestine. The colocalisation 5-HT, hormones hypothesised derive cells different lineages, was also investigated. found throughout but positive were rare rectum. Immunoreactive as common caecum proximal colon they mucosa mRNA gastrin, a can bind some anti-CCK antibodies, only stomach duodenum. Characterisation peptides by extraction, chromatographic separation radioimmunoassay revealed bioactive amidated sulphated forms, including CCK-8 CCK-33. Moreover, CCK-containing bound antibodies target biologically active sulfated form. Colocalisation 5-HT occurred proportion these colocalised colon, where PYY colocalisation. It concluded authentic, active, occurs