Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System
作者： Zhen Liang , Kang Zhang , Kunling Chen , Caixia Gao
DOI: 10.1016/J.JGG.2013.12.001
关键词:
摘要: Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have emerged as powerful tools for genome editing in a variety of species. Here, we report, the first time, targeted mutagenesis Zea mays using TALENs CRISPR/Cas system. We designed five targeting 4 genes, namely ZmPDS, ZmIPK1A, ZmIPK, ZmMRP4, obtained efficiencies up to 23.1% protoplasts, about 13.3% 39.1% transgenic plants were somatic mutations. Also, constructed two gRNAs ZmIPK gene maize at frequencies 16.4% 19.1%, respectively. In addition, system induced mutations Z. protoplasts with (13.1%) similar those (9.1%). Our results show that both can be used modification maize.
cabdirect.org
elsevier.com
researchgate.net 
sci-hub.se 参考文章(28)
Qiwei Shan, Yanpeng Wang, Kunling Chen, Zhen Liang, Jun Li, Yi Zhang, Kang Zhang, Jinxing Liu, Daniel F. Voytas, Xuelian Zheng, Yong Zhang, Caixia Gao, Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs Molecular Plant. ,vol. 6, pp. 1365- 1368 ,(2013) , 10.1093/MP/SSS162
J. Boch, H. Scholze, S. Schornack, A. Landgraf, S. Hahn, S. Kay, T. Lahaye, A. Nickstadt, U. Bonas, Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors Science. ,vol. 326, pp. 1509- 1512 ,(2009) , 10.1126/SCIENCE.1178811
Vladimir Nekrasov, Brian Staskawicz, Detlef Weigel, Jonathan D G Jones, Sophien Kamoun, Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease Nature Biotechnology. ,vol. 31, pp. 691- 693 ,(2013) , 10.1038/NBT.2655
F. Zhang, M. L. Maeder, E. Unger-Wallace, J. P. Hoshaw, D. Reyon, M. Christian, X. Li, C. J. Pierick, D. Dobbs, T. Peterson, J. K. Joung, D. F. Voytas, High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 12028- 12033 ,(2010) , 10.1073/PNAS.0914991107
Vipula K. Shukla, Yannick Doyon, Jeffrey C. Miller, Russell C. DeKelver, Erica A. Moehle, Sarah E. Worden, Jon C. Mitchell, Nicole L. Arnold, Sunita Gopalan, Xiangdong Meng, Vivian M. Choi, Jeremy M. Rock, Ying-Ying Wu, George E. Katibah, Gao Zhifang, David McCaskill, Matthew A. Simpson, Beth Blakeslee, Scott A. Greenwalt, Holly J. Butler, Sarah J. Hinkley, Lei Zhang, Edward J. Rebar, Philip D. Gregory, Fyodor D. Urnov, Precise genome modification in the crop species Zea mays using zinc-finger nucleases Nature. ,vol. 459, pp. 437- 441 ,(2009) , 10.1038/NATURE07992
Zhao Wang, Juan Li, Huang Huang, Gancheng Wang, Mingjun Jiang, Shenyi Yin, Changhong Sun, Hanshuo Zhang, Fengfeng Zhuang, Jianzhong Jeff Xi, An integrated chip for the high-throughput synthesis of transcription activator-like effectors. Angewandte Chemie. ,vol. 51, pp. 8505- 8508 ,(2012) , 10.1002/ANIE.201203597
Qiwei Shan, Yanpeng Wang, Jun Li, Yi Zhang, Kunling Chen, Zhen Liang, Kang Zhang, Jinxing Liu, Jianzhong Jeff Xi, Jin-Long Qiu, Caixia Gao, Targeted genome modification of crop plants using a CRISPR-Cas system. Nature Biotechnology. ,vol. 31, pp. 686- 688 ,(2013) , 10.1038/NBT.2650
Prashant Mali, Luhan Yang, Kevin M Esvelt, John Aach, Marc Guell, James E DiCarlo, Julie E Norville, George M Church, RNA-Guided Human Genome Engineering via Cas9 Science. ,vol. 339, pp. 823- 826 ,(2013) , 10.1126/SCIENCE.1232033
G. Gasiunas, R. Barrangou, P. Horvath, V. Siksnys, Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 15539- 15540 ,(2012) , 10.1073/PNAS.1208507109
Jian-Feng Li, Julie E Norville, John Aach, Matthew McCormack, Dandan Zhang, Jenifer Bush, George M Church, Jen Sheen, Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 Nature Biotechnology. ,vol. 31, pp. 688- 691 ,(2013) , 10.1038/NBT.2654