Regulation of serotonin-facilitated dopamine release in vivo: the role of protein kinase A activating transduction mechanisms.

摘要: Recent neuroanatomical, biochemical, and electrophysiological studies suggest that serotonin (5HT) can modulate dopaminergic function at the level of cell body nerve terminal. The receptor subtypes, regulatory processes, intracellular transduction mechanisms mediating these interactions remain to be characterized. potential involvement cAMP in 5HT-facilitated increases extracellular levels striatal dopamine (DA) was assessed using vivo microdialysis. Local infusion 0.4 nmol 5HT delivered via probes located anterior striata chloral hydrate-anesthetized male rats significantly increased DA approximately 700% basal control levels. Local, intrastriatal either 2 forskolin, rolipram, 100 isobutylmethylxanthine, or 200 dibutyryl 28 +/- 3%, 143 5%, 56 7%, 52 3% above levels, respectively. Additionally, coperfusion any agents with decreased 5HT-facilitory effect on release 50% observed controls. current results a role for second-messenger systems modulating release.