Pendant small functional groups on poly(ϵ-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells.
作者： Min Chen , Yi Zhang , Yan Zhou , Yan Zhang , Meidong Lang
DOI: 10.1016/J.COLSURFB.2015.07.018
关键词:
摘要: Probing stem cell-biomaterial interactions is of great significance in both gaining profound understanding cell biology and advancing tissue regeneration. In the present work, we developed a series poly(ϵ-caprolactone) (PCL) films bearing distinct pendant small functional groups to study effects biomaterial substrate chemistry on behaviors. PCL films, hydroxyl (OH), methyl (CH₃), carboxyl (COOH) amino (NH₂), demonstrated varied surface properties, such as wettability, serum protein adsorption topographical feature. comparison with pristine film, adhesion hMSCs PCL-COOH, PCL-OH PCLCO was significantly promoted cells slightly outgrew PCL-NH₂ PCL-COOH films. Most importantly, tri-lineage differentiation this best osteogenesis achieved PCL-CH₃ supporting superior adipogenic film being most favorable one for chondrogenesis. This highlights critical roles modulating fates MSCs potentially provides practical guidance developing instructive engineering scaffolds.
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