Germ cell differentiation of bone marrow mesenchymal stem cells
作者： Maryam Salem , Tooba Mirzapour , Abolfazl Bayrami , Mohsen Sagha
DOI: 10.1111/AND.13229
关键词:
摘要: Bone marrow mesenchymal stem cells (BM-MSCs) were first cultured under induction of retinoic acid (RA), Sertoli conditioned medium and RA + con (conditioned medium) as treatment groups. The presence was confirmed by immunocytochemistry follicle-stimulating hormone receptor in flow cytometry anti-Gata4 antibody. Cell viability morphology nucleus cytoplasm BM-MSCs evaluated MTT test DAPI staining respectively. expression Oct4, Plzf, Scp3, Caspases 8, 9 3 genes RT-PCR. For increasing the accuracy experiment, Vasa SCP3 investigated quantitatively real-time PCR after 0, 5, 10, 15 days culture. results showed that number apoptotic increased group. apoptosis (Caspases 3, 8 9) also observed this group all Measurement Scp3 RT-PCR positive effects on expression. So, study, a with maximum differentiation minimum DNA fragmentation not
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