Static compression induces zonal-specific changes in gene expression for extracellular matrix and cytoskeletal proteins in intervertebral disc cells in vitro

摘要: Compressive stimuli associated with weight-bearing and loading of the intervertebral disc are believed to be important regulators cell metabolism. In this study, changes in gene expression levels for extracellular matrix cytoskeletal proteins were quantified cells an alginate culture system subjected static unconfined compression (25% compressive strain) after different time periods (2, 18 30 h). Differences observed between anulus fibrosus nucleus pulposus following studied here. Anulus responded mechanical deformation at 30-h point, increasing types I II collagen, aggrecan, biglycan, decorin lumican. contrast, not responsive these any time. Our results also show that cells, but increased vimentin mRNA as well polymerization subunits. The current study illustrate fibrochondrocytes may regulate biosynthesis transcriptional level construct. biological response same is detectable. These differences attributed presence a notochordal population immature here, more diffuse stiff cytoskeleton restrict deformations or shape upon loading.