Wild type human p53 is antiproliferative in SV40-transformed hamster cells.

摘要: The transformation related protein p53 has been implicated in the process of normal cell proliferation and neoplastic transformation. In this study, influence wild type human on was examined. Plasmid constructs encoding various mutant cDNAs, driven by mouse mammary tumor virus (MMTV) promoter linked to dominant biochemical selection marker gpt, were used a colony forming assay employing SV40 transformed HR8 hamster cells. Plasmids drastically reduced number gpt+ colonies obtained after transfection, whereas forms had no effect. Stable clonal lines that constitutively express isolated found have altered growth characteristics (i.e. lower saturation densities, increased doubling times). These findings are consistent with notion could function as suppressor. potential role cycle is discussed.