Functional analysis of GNG2 in human malignant melanoma cells.
作者： Ichiro Yajima , Mayuko Y. Kumasaka , Haruka Tamura , Nobutaka Ohgami , Masashi Kato
DOI: 10.1016/J.JDERMSCI.2012.09.009
关键词:
摘要: Abstract Background Previous studies have revealed that heterotrimeric G protein is composed of a Gα-subunit and Gβγ-dimer correlated with c-Src AKT activities. Our recent study showed reduced γ2 subunit (Gng2/GNG2) expression levels in malignant melanoma cells compared those benign melanocytic both mice humans. At present, however, there no evidence showing an effect Gng2/GNG2 alone on cancer biology. Objective The purpose this was to examine the biological significance GNG2 human cells. Methods Levels proliferation activities signal transduction molecules were examined GNG2-overexpressed -depleted Results Proliferation SK-Mel28 suppressed decreased c-SRC increased p21 Cip/WAF1 level vitro . In contrast, GNG2-depleted A375P enhanced vivo experiment, mean tumor size less than 1/45th control nude at 95 days after inoculation. Conclusion We demonstrated for first time inhibits , suggesting could be novel molecular target therapy.
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sci-hub.se 参考文章(31)
Ichiro Yajima, Masashi Kato, Yuji Naito, Tamio Suzuki, Toshikazu Yoshikawa, Hiro Takahashi, Mayuko Y Kumasaka, Yoko Funasaka, Reduced GNG2 expression levels in mouse malignant melanomas and human melanoma cell lines. American Journal of Cancer Research. ,vol. 2, pp. 322- 329 ,(2012)
Binhua P. Zhou, Yong Liao, Weiya Xia, Yiyu Zou, Bill Spohn, Mien-Chie Hung, HER-2 / neu induces p53 ubiquitination via Akt-mediated MDM2 phosphorylation Nature Cell Biology. ,vol. 3, pp. 973- 982 ,(2001) , 10.1038/NCB1101-973
Oddbjørn Straume, Lisbet Sviland, Lars A. Akslen, Loss of Nuclear p16 Protein Expression Correlates with Increased Tumor Cell Proliferation (Ki-67) and Poor Prognosis in Patients with Vertical Growth Phase Melanoma Clinical Cancer Research. ,vol. 6, pp. 1845- 1853 ,(2000)
Anouk Demunter, Chris De Wolf-Peeters, Hugo Degreef, Marguerite Stas, Joost J. van den Oord, Expression of the endothelin-B receptor in pigment cell lesions of the skin Virchows Archiv. ,vol. 438, pp. 485- 491 ,(2001) , 10.1007/S004280000362
Rosalyn B. Irby, Weiguang Mao, Domenico Coppola, Jimmy Kang, Jean Marc Loubeau, Walter Trudeau, Richard Karl, Donald J. Fujita, Richard Jove, Timothy J. Yeatman, Activating SRC mutation in a subset of advanced human colon cancers Nature Genetics. ,vol. 21, pp. 187- 190 ,(1999) , 10.1038/5971
Dorothy C. Bennett, How to make a melanoma: what do we know of the primary clonal events? Pigment Cell & Melanoma Research. ,vol. 21, pp. 27- 38 ,(2007) , 10.1111/J.1755-148X.2007.00433.X
Masaki Mori, Seigo Kitano, Takeshi Shiraishi, Shinji Tanaka, Kohei Shibata, G-protein γ 7 is down-regulated in cancers and associated with P 27kip1-induced growth arrest Cancer Research. ,vol. 59, pp. 1096- 1101 ,(1999)
M. Bittner, P. Meltzer, Y. Chen, Y. Jiang, E. Seftor, M. Hendrix, M. Radmacher, R. Simon, Z. Yakhini, A. Ben-Dor, N. Sampas, E. Dougherty, E. Wang, F. Marincola, C. Gooden, J. Lueders, A. Glatfelter, P. Pollock, J. Carpten, E. Gillanders, D. Leja, K. Dietrich, C. Beaudry, M. Berens, D. Alberts, V. Sondak, N. Hayward, J. Trent, Molecular classification of cutaneous malignant melanoma by gene expression profiling Nature. ,vol. 406, pp. 536- 540 ,(2000) , 10.1038/35020115
Masashi Kato, Wei Liu, Anwarul A Akhand, Yan Dai, Masaharu Ohbayashi, Toyonori Tuzuki, Haruhiko Suzuki, Ken-Ichi Isobe, Masahide Takahashi, Izumi Nakashima, Linkage between melanocytic tumor development and early burst of Ret protein expression for tolerance induction in metallothionein-I/ret transgenic mouse lines Oncogene. ,vol. 18, pp. 837- 842 ,(1999) , 10.1038/SJ.ONC.1202329
Sangkil Nam, Donghwa Kim, Jin Q. Cheng, Shumin Zhang, Ji-Hyun Lee, Ralf Buettner, Janni Mirosevich, Francis Y. Lee, Richard Jove, Action of the Src family kinase inhibitor, dasatinib (BMS-354825), on human prostate cancer cells. Cancer Research. ,vol. 65, pp. 9185- 9189 ,(2005) , 10.1158/0008-5472.CAN-05-1731