Assignment of PCR markers to river buffalo chromosomes

作者: Hanaa A Oraby , Soheir M El Nahas , H Anna de Hondt , Akmal El Ghor , Mohamed Samad

DOI: 10.1186/1297-9686-30-1-71

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摘要: In the process of developing a buffalo physical map using somatic cell hybrids and cattle gene as template, ten PCR primers designed for four coding genes: F10, FSHB, HBB, CYM six DNA segments: TGLA9, TGLA227, UWCA5, CSSM6, CSSM47 RF131 were tested on panel 47 buffalo-hamster hybrids. F10-TGLA9, FSHB-HBB UWCA5-TGLA227, respectively, found to segregate together forming three syntenic groups. These groups have also been reported in cattle, where they assigned chromosomes BTA 12, 15 18, respectively. Comparative mapping predicts assignment these river BBU 13, 16 © Inra/Elsevier, Paris

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