Putrescine controls the formation of Escherichia coli persister cells tolerant to aminoglycoside netilmicin

摘要: Persisters are suggested to be the products of a phenotypic variability that quasi-dormant forms regular bacterial cells highly tolerant antibiotics. Our previous investigations revealed decrease in antibiotic tolerance Escherichia coli could reached through inhibition key enzymes polyamine synthesis (putrescine, spermidine). We therefore assumed polyamines involved persister cell formation. Data obtained our experiments with polyamine-deficient E. strain demonstrate formation persisters netilmicin is upregulated by putrescine concentration-dependent manner when enter stationary phase. This period also accompanied dissociation initially homogenous subpopulation some fractions differing their levels netilmicin. With three independent experimental approaches, we putrescine-dependent upregulation mediated stimulation rpoS expression. Complementary activity and RpoS results ~ 1000-fold positive effect on