Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells.

摘要: Redox balance has been suggested as an important determinant of "stemness" in embryonic stem cells (ESCs). In this study, we demonstrate that peroxiredoxin (Prx) plays a pivotal role maintenance ESC stemness during neurogenesis through suppression reactive oxygen species (ROS)-sensitive signaling. During neurogenesis, Prx I and Oct4 are expressed mutually dependent manner their expression is abruptly downregulated by excess ROS. Thus, I(-/-) or II(-/-) ESCs, rapid loss can occur due to spontaneous ROS overload, leading active commitment into neurons; however, restored the addition antioxidant inhibitor c-Jun N-terminal kinase (JNK). addition, II appear have tight association with mechanism underlying protection developing teratomas. These results suggest functions protector opposing ROS/JNK cascades neurogenesis. Therefore, our findings implications for understanding involvement enzymes may lead development alternative cell-based therapeutic strategy production high-quality neurons large quantity.