Rapid and efficient homing of human CD34+CD38−/lowCXCR4+stem and progenitor cells to the bone marrow and spleen of NOD/SCID and NOD/SCID/B2mnull mice

摘要: Stem cell homing into the bone microenvironment is first step in initiation of marrow-derived blood cells. It reported that human severe combined immunodeficient (SCID) repopulating cells home and accumulate rapidly, within a few hours, marrow spleen mice previously conditioned with total body irradiation. Primitive CD34(+)CD38(-/low)CXCR4(+) capable engrafting primary secondary recipient selectively homed to spleen, whereas CD34(-)CD38(-/low)Lin(-) were not detected. Moreover, freshly isolated CD34(+)CD38(+/high) did home, vivo stimulation granulocyte colony-stimulating factor as part mobilization process, or vitro stem for 2 4 days, potentiated capabilities cytokine-stimulated CD34(+)CD38(+) Homing enriched CD34(+) was inhibited by pretreatment anti-CXCR4 antibodies. primitive also response gradient stromal cell-derived 1 (SDF-1), directly injected nonirradiated NOD/SCID mice. antibodies major integrins VLA-4, VLA-5, LFA-1. Pertussis toxin, an inhibitor signals mediated Galpha(i) proteins, SDF-1-mediated transwell migration but adhesion blocked chelerythrine chloride, broad-range protein kinase C inhibitor. This study reveals rapid efficient murine integrin depends on activation signal transduction pathway SDF-1.