Simultaneous phenotypic and genetic characterization of single circulating tumor cells from colon cancer patients.

摘要: Since circulating tumor cells (CTCs) have metastatic potential, their genetic and phenotypic characteristics could provide crucial information to establish the most effective therapy. We assessed clinical utility of a methodology that allows simultaneous analysis CTC phenotype genotype in colon cancer patients and, addition, whether this complementary obtained by primary biopsy. Thirty-three non-metastatic (stages 0-III) 9 healthy donor samples were evaluated. All peripheral blood (10 ml) analyzed cytokeratin immunomagnetic enrichment. Eight immunocytochemistry 25 FICTION technique for expression chromosome 17 ERBB2 gene status. A further study was carried out one patient who showed heterogeneity chromosomal abnormalities. HER2 protein on CTCs FISH patient. Our results show 9.09% had cytokeratin-positive (CK+/CTCs blood). One abnormalities two different CK patterns CTCs: CK+/CTCs CK-/CTCs. Furthermore, 63.33% these overexpressed while diploid did not express protein. describe patients, which may essential select might benefit from specific