The use of protein array to identify targetable receptor tyrosine kinases for treatment of human colon cancer

摘要: Several studies have reported that activated receptor tyrosine kinases (RTKs) are highly expressed in colon cancer and may promote tumor growth survival. However, there is little information available as to the function signaling of RTKs cancers. In present study, we performed protein array technology determine expression status various compared normal colonic cells tissues. Of 42 different phospho-RTKs, 5 (ErbB2, FGFR1, FGFR2a, FGFR3 MSPR) were Caco-2, SW480, WiDr, Lovo cell lines cancerous order effect inhibition RTKs, especially ErbB2, athymic nude mice bearing xenograft tumors treated with ErbB2-targeting drug trastuzumab alone, or combination 5-Fluorouracil (5-FU). Similar treatment 5-FU suppressed cancer. Combination therapy inhibited significantly alone alone. addition, also analyzed by phospho-MAPK array. The activity Akt3/PKBgamma was trastuzumab, indicating inhibit through ErbB2-Akt3/PKBgamma signaling. These data demonstrate ErbB2 could be an important candidate for addition might augment clinical response patients. Therefore, analysis phospho-RTK a useful tool identify novel therapies individual patients