Dephosphorylation and reactivation of phosphorylated purified ox-kidney branched-chain dehydrogenase complex by co-purified phosphatase
作者： Hasmukh R. Fatania , Philip A. Patston , Philip J. Randle
DOI: 10.1016/0014-5793(83)80585-7
关键词:
摘要: The branched-chain 2 oxoacid dehydrogenase complex has been purified from well-washed ox-kidney mitochondria together with kinase. was inactivated and phosphorylated by ATP in about 5 min at 30 degrees C. After hydrolysis of a contaminating ATPase (5-10 min) the dephosphorylated reactivated. Dephosphorylation reactivation were linearly correlated. Reactivation dependent upon Mg2+ (K0.5 greater than 1 mM) inhibited completely 50 mM fluoride. dephosphorylation are attributed to mitochondrial phosphatase.
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