Activity state of the branched chain α-ketoacid dehydrogenase complex in heart, liver, and kidney of normal, fasted, diabetic, and protein-starved rats
作者： Sarah E. Gillim , Ralph Paxton , George A. Cook , Robert A. Harris
DOI: 10.1016/S0006-291X(83)80119-3
关键词: Liver and kidney 、 In vivo 、 Biochemistry 、 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) 、 Endocrinology 、 Internal medicine 、 Dehydrogenase complex 、 Diabetes mellitus 、 Kidney 、 Dehydrogenase 、 Biology 、 Enzyme
摘要: The proportion of active (unphosphorylated) branched chain alpha-ketoacid dehydrogenase was determined in tissues from rats different metabolic states. Hearts normal, high-protein, and low-protein fed contained about 45% the enzyme form. Only 10-20% hearts fasted diabetic rats. Virtually all liver form fed, fasted, high-protein animals. Protein starved rats, however, exhibited a dramatic decrease both % total amount enzyme. Kidneys 70-80% kidney decreased protein but less dramatically than liver. Covalent modification is concluded to be important for vivo regulation complex.
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