A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles

摘要: The 5' ends of all newly synthesized single-stranded (s1) DNA genomes the autonomous parvovirus minute virus mice are covalently linked to major virally coded nonstructural protein NS-1, but later in infection this association is disrupted, giving rise an abbreviated form designated s2. Both s1 and s2 forms encapsidated migrate velocity gradients as 110S particles, and, such, both appear be infectious. Most virions released from A9 cells NS-1 molecules located on outside virion where they accessible antibodies enzymes. These polypeptides cleaved by nucleolytic or proteolytic digestion, which can occur either culture medium upon subsequent entry into further host cells. Since cleavage minimized blocking viral reentry, it likely that most processing occurs after cell. Incoming rapidly converted when used infect new cells, vitro removal with proteases nucleases fails influence infectivity particles under normal conditions. Limited proteolysis trypsin demonstrates via its amino-terminal domain. Analysis indicates there approximately 24 externally nucleotides linking 5.1-kilobase nuclease-resistant core virion.