Comprehensive Expression Profiling and Functional Network Analysis of p53-Regulated MicroRNAs in HepG2 Cells Treated with Doxorubicin
作者： Yalan Yang , Wenrong Liu , Ruofan Ding , Lili Xiong , Rongkun Dou
DOI: 10.1371/JOURNAL.PONE.0149227
关键词:
摘要: Acting as a sequence-specific transcription factor, p53 tumor suppressor involves in variety of biological processes after being activated by cellular stresses such DNA damage. In recent years, microRNAs (miRNAs) have been confirmed to be regulated several cancer types. However, it is still unclear how miRNAs orchestrate their regulation and function network activation hepatocellular carcinoma (HCC). this study, we used small RNA sequencing systematic bioinformatic analysis characterize the regulatory networks differentially expressed HepG2. Here, 33 significantly (12 up-regulated 21 down-regulated) were detected between doxorubicin-treated untreated HepG2 cells two replicates for 8 reported previously associated with HCC. Gene ontology (GO) KEGG pathway enrichment showed that 87.9% (29 out 33) 90.9% (30 p53-regulated involved p53-related pathways low p-value, respectively. Remarkably, 18 identified contain binding sites around start (TSSs). Finally, comprehensive p53-miRNA constructed analyzed. These observations provide new insight into co-regulatory context
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