Immunochromatographic strip development for ultrasensitive analysis of aflatoxin M1

摘要: A specific monoclonal antibody against aflatoxin (AF) M1 was prepared by a duo-immunogen immunization strategy. After two immunizations of Balb/c mice with AFB1 keyhole limpet hemocyanin (KLH) conjugates and followed five subsequent AFM–KLH, the secreting AFM1 were selected for cell fusion 7C6-H1 line obtained to produce antibody. The 50% inhibition rate calculated as 0.034 ± 0.002 μg L−1. anti-AFM1 characterized high affinity (1.28 × 109 mol L−1) low cross-reactivity related AFs (<5%). With competitive format, an immunochromatographic strip developed using AFB1–bovine serum albumin immobilized antigen labeled gold nanoparticles tracers. Without any sample preparation, could be directly applied detect contamination in liquid milk. minimum cut-off concentrations 0.2 L−1 1.6 kg−1 milk powdered milk, respectively, assessment naked eye. Detection real samples spiked indicated potential this routine monitoring.