Metformin inhibits proliferation and migration of glioblastoma cells independently of TGF-β2.

摘要: ABSTRACTTo this day, glioblastoma (GBM) remains an incurable brain tumor. Previous research has shown that metformin, oral anti-diabetic drug, may decrease GBM cell proliferation and migration especially in tumor initiating cells (BTICs). As transforming growth factor β 2 (TGF-β2) been reported to promote high-grade glioma is inhibited by metformin other tumors, we explored whether directly interferes with TGF-β2-signaling. Functional investigation of primary BTICs after treatment metformin+/−TGF-β2 revealed doses as low 0.01 mM thrice a day were able inhibit susceptible lines, whereas was impacted only at higher doses. Known cellular mechanisms such increased lactate secretion, reduced oxygen consumption activated AMPK-signaling, could be confirmed. However, TGF-β2 did not act functional antagonists, but both rather ...