Tracking T-cell immune reconstitution after TCRαβ/CD19-depleted hematopoietic cells transplantation in children.
作者： I V Zvyagin , I Z Mamedov , O V Tatarinova , E A Komech , E E Kurnikova
DOI: 10.1038/LEU.2016.321
关键词:
摘要: αβT-cell-depleted allogeneic hematopoietic cell transplantation holds promise for the safe and accessible therapy of both malignant non-malignant blood disorders. Here we employed molecular barcoding normalized T-cell receptor (TCR) profiling to quantitatively track immune reconstitution after TCRαβ-/CD19-depleted in children. We demonstrate that seemingly early αβT-cell counts 2 months is based on only several hundred rapidly expanded clones originating from non-depleted graft cells. In further months, frequency these hyperexpanded declines, 1 year observed TCRβ diversity are mostly provided by newly produced T also high at day 60 some patients determined recipient cells intrathymic progenitors survived conditioning regimen. Our results indicate efforts optimization protocols should be directed toward providing more efficient defense first transplantation.
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