Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas

摘要: Nineteen specimens from primary human malignant mesotheliomas obtained 19 patients were screened for activating point mutations in the oncogenes N-ras and CDK4 by combined RFLP-PCR/SSCP analysis. In addition, all tumours deletions tumour suppressor genes p53, p16 INK4a (CDKN2A) p14 ARF (exon-18) multiplex-PCR/SSCP No found N-ras, p53 CDK4. Three displayed homozygous deletion (co-deletion of exons 1, 2 3) . One them additional (exon-If)). Two silent polymorphisms 3 tumours. Our preliminary data indicate that disarrangement Rb1 pathway may be involved mesothelioma formation.