Monoclonal Anti-MAGE-3 CTL Responses in Melanoma Patients Displaying Tumor Regression after Vaccination with a Recombinant Canarypox Virus

摘要: We have analyzed the T cell responses of HLA-A1 metastatic melanoma patients with detectable disease, following vaccination a recombinant ALVAC virus, which bears short MAGE-1 and MAGE-3 sequences coding for antigenic peptides presented by HLA-A1. To evaluate anti-MAGE CTL responses, we resorted to stimulation blood lymphocytes under limiting dilution conditions, followed tetramer analysis cloning tetramer-positive cells. The clones were tested their specific lytic ability TCR obtained. Four who showed tumor regression analyzed, an anti-MAGE-3.A1 response was observed in three these patients. Postvaccination frequencies 3 x 10(-6), 10(-3), 10(-7) CD8 cells, respectively. These monoclonal. No anti-MAGE-1.A1 observed. results indicate that, like peptide immunization, immunization produces monoclonal responses. They also suggest that low-level antivaccine can initiate process. Taken together, our or shows degree correlation between regression, but firm conclusions will require larger numbers.