Rapid quantification of astilbin in rat plasma by liquid chromatography-tandem mass spectrometry and its application to pharmacokinetic study
作者： Lei Yin , Yun-hui Zhang , Sen Zhao , Long-mei Cheng , Mei-yun Shi
DOI: 10.1007/S40242-013-3166-8
关键词:
摘要: Astilbin is a potential immunosuppressive agent with minor cytotoxicity. Its oral bioavailability supposed to be rather low and therefore sensitive analytical method required for its pharmacokinetic study after administration. A simple, rapid liquid chromatography-tandem mass spectrometry(LC-MS/MS) was developed validated the determination of astilbin in rat plasma. Plasma samples were subjected liquid-liquid extraction ethyl acetate separated by reversed phase high performance chromatography(HPLC) methanol-0.01%(volume fraction) formic acid(50:50, volume ratio) as mobile phase. Quantitive achieved on negative LC-MS/MS multiple reaction moitoring transitions m/z 449.1→150.9(quantifier) 449.1→284.9(qualifier) 128.9→42.0 internal standard(IS). lower limit quantification(LLOQ) ng/mL within short cycle time 3.4 min. The successfully applied involving intravenous administrations 6 mg/kg six rats.
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