Renal cytokine responses in acute Escherichia coli pyelonephritis in IL‐6‐deficient mice

摘要: The aim of this study was to investigate the influence IL-6 on mortality, bacterial growth and cytokine expression in experimental acute pyelonephritis. Female IL-6-deficient mice their wild-type counterparts, 8–10 weeks old, were infected with Escherichia coli CFT 073 or injected NaCl 0·9% (w/v) via urethra thereafter obstructed for 6 h. Animals killed at 48 h, days 8 renal levels assessed each time point. We found that had increased mortality extensive day 6, compared (P < 0·05) histopathological changes generally more severe widespread mice. Peak mRNA IL-1β, IL-4, IL-10, IL-12 interferon-gamma (IFN-γ) occurred h after infection both knock out Transforming factor-beta (TGF-β) also peaked E. coli-infected mice, while strain TGF-β protein significantly lower than 0·0008 P 0·03, respectively) remained stationary throughout period. displayed a similar decrease 0·02). When splenocytes from incubated murine recombinant IL-6, those No increase observed when same doses rIL-6. therefore conclude plays an important role clearance directly influences demonstrate urethral obstruction per se induces magnitude which is decreased