A New Family of Cdc42 Effector Proteins, CEPs, Function in Fibroblast and Epithelial Cell Shape Changes
作者： Dianne Snow Hirsch , Dana M. Pirone , Peter D. Burbelo
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摘要: Cdc42, a Rho GTPase, regulates the organization of actin cytoskeleton by its interaction with several distinct families downstream effector proteins. Here, we report identification four new Cdc42-binding proteins that, along MSE55, constitute family These molecules, designated CEPs, contain three regions homology, including Cdc42 binding domain and two unique domains called CI CII. Experimentally, have verified that CEP2 CEP5 bind Cdc42. Expression CEP2, CEP3, CEP4, in NIH-3T3 fibroblasts induced pseudopodia formation. Fibroblasts coexpressing dominant negative or expressing Cdc42/Rac interactive mutant did not induce In primary keratinocytes, CEP2- CEP5-expressing cells showed reduced F-actin localization at adherens junctions an increase thin stress fibers extended length cell body. Keratinocytes CEPs also altered vinculin distribution loss E-cadherin from junctions. Similar effects were observed keratinocytes constitutively active but seen CEP2. results suggest act to filament assembly leading shape changes.
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