Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum

摘要: Schistosomes are the causative agents of schistosomiasis, one most prevalent and serious parasitic diseases that currently infects ∼200 million people worldwide. Schistosome excretory/secretory (ES) proteins have been shown to play important roles in modulating mammalian host immune systems. In our current study, we performed a global proteomics identification ES from adult worms Schistosoma japonicum, three major schistosome species. Our results unambiguously identified 101 proteins, including 53 putatively secreted proteins. By quantitative analysis, revealed fatty acid-binding protein as constituent vitro proteome. Strikingly heat shock HSP70s, HSP90, HSP97 constituted largest family proteome, implying central role for these immunomodulation host-parasite relationship. Other S. japonicum included actins, 14-3-3, aminopeptidase, enolase, glyceraldehyde-3-phosphate dehydrogenase, some which considered viable vaccine candidates therapeutic targets. A comparison with previous studies suggests 48.5% common other parasite products, indicating molecular mechanisms involved evading response may be conserved across different parasites. Interestingly seven antimicrobial CAP18, immunoglobulins, complement component, were among products likely originating tegument or gut, innate acquired systems could defend against invasion. present study represents first attempt at profiling provides an insight into interactions, establishes resource development diagnostic vaccines control schistosomiasis.