Method for methylation-selective amplification

作者: Dimo Dr. Dietrich

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摘要: Aspects of the invention relate to a method for methylation selective amplification. The comprising DNA treatment, wherein cytosine is converted uracil, uracil sulfonate or another base having different binding behavior than cytosine, while methylated remains unchanged, and amplification treated in presence at least one restriction enzyme, said enzyme digesting product derived either from unmethylated during kit performing inventive method. also use methods kits.

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