Biodegradable polymer film as a source for formation of human fetal retinal pigment epithelium spheroids.

摘要: PURPOSE. To evaluate the attachment of human fetal retinal pigment epithelial (HFRPE) cells to a biodegradable polymer film with subsequent formation spheroids in vitro. METHODS. Ten films different compositions were examined for their physical properties and ease manipulation under dissecting microscope. The most suitable handling characteristics was chosen, purely isolated sheet HFRPE attached it. purity assessed by pigmentation expression cytokeratin. Proliferation incorporation 5-bromo-2'-deoxyuridine (BrdU). Cellular structure analyzed light electron microscopes, functional capability evaluated rod outer segment (ROS) phagocytosis. RESULTS. composition 50:50 poly (DL-lactide) (PLA)/poly (DL-lactide-co-glycolide) (PLG) an inherent viscosity 1.03 dl/g found be Sheets within 48 hours began form spheroids. All pigmented expressed They possessed cuboidal morphology, numerous apical microvilli, no sign dedifferentiation. produced extracellular matrix (collagen filaments) on basal side, filling cavities film. subsequently proliferated, incorporated BrdU, migrated onto culture plate monolayers, phagocytized ROS. CONCLUSIONS. Biodegradable can used as scaffold adhesion Spheroids represent source high density well-differentiated that are easy transfer. Furthermore, membrane makes it additional applications.