Plasma-polymerized allylamine film used as a new solid phase in immunoradiometric assay (IRMA): effect of antibody (F(ab')2 fragment) concentration on dose response in two-site IRMA.

摘要: Allylamine (ALAM) film was plasma-polymerized on a flat glass (referred to as ALAM(GLA): GLA refers plate) for use solid phase in two-site immunoradiometric assay (two-site IRMA). Adsorption of F(ab')2 anti-human immunoglobulin G [formula: see text] ALAM(GLA) larger than adsorption polyvinyl chloride plate PVC). Contrary the expectation that dose response human IgG (hIgG) ALAM (GLA) better PVC, responses both phases were same. This phenomenon independent molecular size antigen (Ag) (hIgG or Fc fragment hIg (hIgG-Fc)) and also reaction with protein A (pA). Because direct measurements binding 125I-labeled hIgG (hIgG*) hIgG-Fc (hIgG-Fc*) showed no difference between not due second step system IRMA (an Ag--Ab (Ab antibody)). These results indicated first (the phase). When concentration immobilized lowered, significant increase observed ALAM(GLA).