Specific and sensitive plate assay for bacterial lipases.
作者： G Kouker , K E Jaeger
DOI: 10.1128/AEM.53.1.211-213.1987
关键词:
摘要: A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes formation of orange halos around colonies visible upon UV irradiation. The logarithm activity from cell-free culture supernatants linearly correlated with diameter halos, thereby allowing quantitation activities ranging 1 30 nkat.
sci-hub.st 参考文章(10)
M. A. Collins, B. W. Hammer, The Action of Certain Bacteria on Some Simple Tri-Glycerides and Natural Fats, as Shown by Nile-Blue Sulphate. Journal of Bacteriology. ,vol. 27, pp. 473- 486 ,(1934) , 10.1128/JB.27.5.473-486.1934
Nina Peled, Michael C. Krenz, A new assay of microbial lipases with emulsified trioleoyl glycerol Analytical Biochemistry. ,vol. 112, pp. 219- 222 ,(1981) , 10.1016/0003-2697(81)90284-0
Gonzalo Sierra, A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates Antonie van Leeuwenhoek. ,vol. 23, pp. 15- 22 ,(1957) , 10.1007/BF02545855
J. Karnetová, J. Matějů, T. Řezanka, P. Procházka, M. Nohýnek, J. Rokos, Estimation of lipase activity by the diffusion plate method Folia Microbiologica. ,vol. 29, pp. 346- 347 ,(1984) , 10.1007/BF02875969
Mechtild Höfelmann, Ruth Kittsteiner-Eberle, Peter Schreier, Ultrathin-layer agar gels: A novel print technique for ultrathin-layer isoelectric focusing of enzymes Analytical Biochemistry. ,vol. 128, pp. 217- 222 ,(1983) , 10.1016/0003-2697(83)90367-6
Robert G. Jensen, Detection and determination of lipase (acylglycerol hydrolase) activity from various sources Lipids. ,vol. 18, pp. 650- 657 ,(1983) , 10.1007/BF02534677
J. A. Alford, Elsie E. Steinle, A double layered plate method for the detection of microbial lipolysis. Journal of Applied Microbiology. ,vol. 30, pp. 488- 494 ,(1967) , 10.1111/J.1365-2672.1967.TB00326.X
H. Wand, A. Gabert, H. Uhlig, Einfache Methode zum Nachweis mikrobieller Lipase Journal of Basic Microbiology. ,vol. 20, pp. 291- 293 ,(1980) , 10.1002/JOBM.19800200409
Robert D. Mackenzie, Thomas R. Blohm, Edward M. Auxier, Andrew C. Luther, Rapid colorimetric micromethod for free fatty acids. Journal of Lipid Research. ,vol. 8, pp. 589- 597 ,(1967) , 10.1016/S0022-2275(20)38880-5
Shabanova Ea, Kazakov Ga, Kuimova Tf, Rapid selection of microorganisms producing extracellular lipases Microbiology. ,vol. 44, pp. 365- 366 ,(1975)