The uptake and development of vaccinia virus in strain L cells followed with labeled viral deoxyribonucleic acid.

摘要: Vaccinia virus which had its DNA labeled with thymidine-H3 was purified and used as inoculum for L cells growing in suspension. Samples taken over an 8-hour period after infection were studied by light electron microscopic autoradiography. Within 20 minutes of being up at the cell membrane phagocytic vesicles, outer coat vaccinia becomes disrupted core containing passes into cytoplasmic matrix. 1 hour inoculation material out cores zones viroplasm, where or remnants are gathered label more concentrated 3 hours inoculation. Most is conserved viroplasm areas during remainder experiment. However, 6 a very small proportion progeny cytoplasm, morphologically distinct from inoculum, has associated it material, perhaps derived inoculum.