Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor

摘要: A full length cDNA encoding the growth hormone receptor (GHR) of Chinese sturgeon was cloned in order to investigate mechanism regulating sturgeon. The open reading frame (csGHR) encodes a trans-membrane protein 611 amino acids containing all characteristic motifs GHR. By sequence alignment, substitutions acid residues highly conserved other species were identified. Using CHO cell culture system, function csGHR and biological significance substitution examined. promoter serine protease inhibitor 2.1 (Spi2.1) trans-activated upon stimulation seabream GH (sbGH) csGHR-expressing cells. Furthermore, cells stably expressing stimulated proliferate by sbGH. In agreement with our previous report, hormone-binding (csGHBP) detected medium csGHR. Mutation Asp residue ligand binding motif Glu significantly enhanced csGHR’s function, whereas mutation Ala decreased its function. results demonstrated that csGHBP could be generated through proteolysis These findings might provide new insights into thoroughly understanding regulatory growth.