Requirement of Tyrosine Residues 333 and 338 of the Growth Hormone (GH) Receptor for Selected GH-stimulated Function
作者： Peter E. Lobie , Giovanna Allevato , Jens H. Nielsen , Gunnar Norstedt , Nils Billestrup
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摘要: We have examined the involvement of tyrosine residues 333 and 338 growth hormone (GH) receptor in cellular response to GH. Stable Chinese hamster ovary (CHO) cell clones expressing a with at position substituted for phenylalanine (CHO-GHR1-638Y333F, Y338F) were generated by cDNA transfection. Compared wild type Y333F,Y338F mutant possessed normal high affinity ligand binding, internalization, ligand-induced down-regulation. GH activation mitogen-associated protein kinase was also similar CHO number. However, two GH-regulated events (lipogenesis, synthesis) deficient receptor. In contrast, transcriptional regulation (as evidenced chloramphenicol acetyltransferase expression driven GH-responsive region SPI 2.1 gene) not affected substitution. Thus we provide first experimental evidence that specific are required selected responses
sci-hub.se 参考文章(60)
A.C. Greenlund, M.A. Farrar, B.L. Viviano, R.D. Schreiber, Ligand-induced IFN gamma receptor tyrosine phosphorylation couples the receptor to its signal transduction system (p91). The EMBO Journal. ,vol. 13, pp. 1591- 1600 ,(1994) , 10.1002/J.1460-2075.1994.TB06422.X
X Wang, M.D. Uhler, N Billestrup, G Norstedt, F Talamantes, J.H. Nielsen, C Carter-Su, Evidence for association of the cloned liver growth hormone receptor with a tyrosine kinase. Journal of Biological Chemistry. ,vol. 267, pp. 17390- 17396 ,(1992) , 10.1016/S0021-9258(18)41938-2
X. Wang, C. Möller, G. Norstedt, C. Carter-Su, Growth hormone-promoted tyrosyl phosphorylation of a 121-kDa growth hormone receptor-associated protein. Journal of Biological Chemistry. ,vol. 268, pp. 3573- 3579 ,(1993) , 10.1016/S0021-9258(18)53732-7
W. L. Farrar, H. Rui, L. Dasilva, R. A. Kirken, O. M. Z. Howard, Growth signaling and JAK2 association mediated by membrane-proximal cytoplasmic regions of prolactin receptors Journal of Biological Chemistry. ,vol. 269, pp. 18267- 18270 ,(1994) , 10.1016/S0021-9258(17)32296-2
L.A. Winston, P.J. Bertics, Growth hormone stimulates the tyrosine phosphorylation of 42- and 45-kDa ERK-related proteins. Journal of Biological Chemistry. ,vol. 267, pp. 4747- 4751 ,(1992) , 10.1016/S0021-9258(18)42895-5
P. Colosi, K. Wong, S.R. Leong, W.I. Wood, Mutational analysis of the intracellular domain of the human growth hormone receptor. Journal of Biological Chemistry. ,vol. 268, pp. 12617- 12623 ,(1993) , 10.1016/S0021-9258(18)31433-9
Hichem Mertani, Gunnar Norstedt, Orlando Moralesbustos, Peter E. Lobie, Michael J. Waters, Gerard Morel, Receptor-mediated nuclear translocation of growth hormone. Journal of Biological Chemistry. ,vol. 269, pp. 21330- 21339 ,(1994) , 10.1016/S0021-9258(17)31966-X
C. Carter-Su, G. S. Campbell, J. A. Vanderkuur, Xueyan Wang, Liying Zhang, G. Norstedt, N. Billestrup, G. Allevato, Domains of the growth hormone receptor required for association and activation of JAK2 tyrosine kinase Journal of Biological Chemistry. ,vol. 269, pp. 21709- 21717 ,(1994) , 10.1016/S0021-9258(17)31863-X
G.S. Campbell, L Pang, T Miyasaka, A.R. Saltiel, C Carter-Su, Stimulation by growth hormone of MAP kinase activity in 3T3-F442A fibroblasts. Journal of Biological Chemistry. ,vol. 267, pp. 6074- 6080 ,(1992) , 10.1016/S0021-9258(18)42663-4
D.J. Meyer, G.S. Campbell, B.H. Cochran, L.S. Argetsinger, A.C. Larner, D.S. Finbloom, C. Carter-Su, J. Schwartz, Growth hormone induces a DNA binding factor related to the interferon-stimulated 91-kDa transcription factor Journal of Biological Chemistry. ,vol. 269, pp. 4701- 4704 ,(1994) , 10.1016/S0021-9258(17)37599-3