Isolation and recombination of ovine lutropin subunits with complete recovery of biological activity.
作者： M Ascoli , W K Liu , D N Ward
DOI: 10.1016/S0021-9258(19)63344-2
关键词:
摘要: Abstract Based on the results obtained in previous paper (Liu, W.-K., Ascoli, M., and Ward, D. N. (1977) J. Biol. Chem. 252, 5274-5279) we have devised a method that permits isolation of subunits ovine lutropin (oLH) their "native" form. The hormone was dissociated by guanidine HCl, separated salt precipitation (Sairam, M. R., Li, C. H. (1974) Arch. Biochem. Biophys. 165, 709-714). present differs significantly from Sairam Li all operations are carried out at pH 5.0 or higher, condition shown to be crucial maintain integrity isolated subunits. yield 75 85%. exceed 95% purity (i.e. contamination with other subunit intact is less than 5%), migrate as single bands sodium dodecyl sulfate-polyacrylamide gels, show only NH2-terminal heterogeneity expected native hormone. Recombination can accomplished 85% (with respect mass) full recovery biological activity.
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