Low-density lipoprotein receptor-mediated endocytosis of PEGylated nanoparticles in rat brain endothelial cells.
作者： H. Ryoung Kim , S. Gil , K. Andrieux , V. Nicolas , M. Appel
DOI: 10.1007/S00018-007-6390-X
关键词:
摘要: Poly(methoxypolyethyleneglycol cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles have demonstrated their capacity to diffuse through the blood-brain barrier after intravenous administration. However, mechanism of transport these into brain has not yet been clearly elucidated. The development a model rat endothelial cells (RBEC) in culture allowed investigations this mechanism. A study intracellular trafficking by cell fractionation and confocal microscopy showed that are internalized endocytic pathway. Inhibition caveolae-mediated pathway preincubation with filipin nystatin did modify cellular uptake nanoparticles. In contrast, chlorpromazine NaN(3) pretreatment, which interferes clathrin energy-dependent endocytosis, caused significant decrease nanoparticle internalization. Furthermore, experiments preincubated apolipoprotein E blocking low-density lipoprotein receptors (LDLR) suggested LDLR-mediated was involved endocytosis PEGPHDCA RBEC.
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