Infectivity of the DNA from four isolates of JC virus.

摘要: The infectivity of JC virus DNA was demonstrated in its most permissive cell culture, primary human fetal glial cells. amount observed these heterogeneous cultures varied considerably between batches Contrary to results obtained with the papovaviruses simian 40 and BK virus, calcium technique (F. L. Graham A. J. van der Eb, Virology 52:456--467, 1973) found be more efficient at promoting than DEAE-dextran method (J. H. McCutchan S. Pagano, Natl. Cancer Inst. 41:351--357, 1968): maximum titers 4 x 10-(4) 6 10(3) fluorescent units per microgram DNA, respectively. These values represent an approximate recovery from 0.02 0.14%. Comparisons DNAs four isolates which differed their degrees heterogeneity did not reveal significant differences. infectious lung kidney