The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand

摘要: Dengue represents a major public health problem in Thailand, with all four viral serotypes co-circulating. virus serotype 4 (DENV-4) is the least frequently sampled serotype, although one that often associated hemorrhagic fever during secondary infection. To determine evolutionary forces shaping genetic diversity of DENV-4, and particularly whether its changing prevalence could be attributed to instances adaptive evolution genome, we undertook large-scale molecular epidemiological analysis DENV-4 Bangkok, using both E gene complete coding region sequences. This revealed extensive within single locality at time, including discovery new divergent genotype as well pattern continual lineage turnover. We also recorded highest average rate change for this 1.072 x 10(-3) nucleotide substitutions per site, year. However, despite abundant variation, there was no evidence any gene, codon, or nonsynonymous substitution observed NS2A. Consequently, rapid turnover lineages through time most likely consequence high deleterious mutation genome coupled seasonal fluctuations size vector population.