Karyophilic properties of Semliki Forest virus nucleocapsid protein.

摘要: Semliki Forest virus capsid (C) protein molecules (Mr, 33,000) can be introduced efficiently into the cytoplasm of various target cells by electroporation, liposome, and erythrocyte ghost-mediated delivery (M. Elgizoli, Y. Dai, C. Kempf, H. Koblet, M.R. Michel, J. Virol. 63:2921-2928, 1989). Here, we show that transferred C partition rapidly from cytosolic compartment nucleus. Transport nucleus was reversibly arrested metabolic inhibitors, indicating transfer process is energy dependent. Fractionation isolated nuclei revealed delivered preferentially associates with nucleoli. This finding confirmed morphological studies, showing in an vitro system containing ATP accumulated rhodamine-labeled their Furthermore, this assay system, lectin wheat germ agglutinin prevented through nuclear pores. These results are agreement our observation nucleoli contain measurable amounts newly synthesized as early 5 h after infection SFV. Thereafter, nucleolar-associated increased progressively during course infection.